ABSTRACT
A simple, sensitive, and inexpensive high performance liquid chromatographic method has been developed for simultaneous determination of ofloxacin and ornidazole in pharmaceutical formulations. Chromatographic separation was achieved on a BDS-C[18]-Hypersil column [250 mm x 4.6 mm i.d., 10 microm]. Mobile phase was 80% water [containing 0.55 ml/L of triethylamine as peak modifier] and 20% acetonitrile; final pH was adjusted to 3.0 with orthophosphoric acid. Detection was done at 284 nm. Response was a linear function of concentration in the range 1-20 micro g/ml for ofloxacin and 2.5-50 microg/ml for ornidazole; the correlation coefficients were 0.9998 and 0.9995, respectively. The limit of detection were 0.01 and 0.02 microg/ml for ofloxacin and ornidazole respectively, where as limit of quantitation were 0.05 and 0.1 micro g/ml. The accuracy result for ofloxacin and ornidazole at eighty percent drug [80%], hundred percent [100%], and one hundred and twenty percent [120%] were ranged from 99.6-100.9%. The inter- and intra-day precision was less than 1%. Total elution time for the two components was less than 9 min